A Comprehensive Guide on How to Reconstitute Tesamorelin 10 mg

October 11, 2025 •

4 min read

Tesamorelin is a synthetic peptide that stimulates the release of growth hormone, and while FDA-approved versions come in smaller vials, 10 mg formulations are commonly found as compounded or research-grade peptides. Knowing how to reconstitute tesamorelin 10 mg correctly is crucial for ensuring potency, safety, and proper administration.

Quick Summary

This guide provides detailed instructions for reconstituting 10 mg of tesamorelin, a process specific to non-FDA-approved compounded or research formulations. It covers the necessary materials, proper aseptic technique, mixing procedures, and vital safety considerations for storage and administration.

Key Points

Verify Formulation: A 10 mg tesamorelin vial is typically a compounded or research-grade peptide, not an FDA-approved product like Egrifta.
Use Bacteriostatic Water: Reconstitute the 10 mg vial using bacteriostatic water for injection, which contains a preservative suitable for multi-dose use.
Mix Gently: Do not shake the vial; instead, roll it gently between your hands for about 30 seconds until the powder is fully dissolved to prevent peptide damage.
Store Properly: After mixing, store the reconstituted solution in the refrigerator (2–8°C) and use it within 30 days. Keep it protected from light.
Practice Aseptic Technique: Ensure your hands and all materials are clean, and sterilize vial stoppers with alcohol pads before use to maintain sterility.
Consult for Dosage: Always consult your healthcare provider or prescribing clinic for specific dosage recommendations and how to measure them based on your reconstitution volume.

Important Note on Tesamorelin Formulations

It is critical to understand that there are no FDA-approved 10 mg tesamorelin products. The brand-name drug, Egrifta, is approved in different dosages (2 mg for EGRIFTA SV and 11.6 mg for EGRIFTA WR) for HIV-related lipodystrophy. This guide specifically addresses the reconstitution of 10 mg tesamorelin, which is typically a compounded or research-grade peptide and requires the use of bacteriostatic water for multi-dose viability. Always follow the specific instructions from your prescribing clinic or research provider.

Materials Needed for Reconstitution

Before beginning, gather all required materials to ensure a smooth and sterile process:

One vial of tesamorelin (10 mg) in lyophilized (freeze-dried) powder form.
Bacteriostatic water for injection (the recommended diluent for multi-use peptides).
Syringe for mixing (e.g., 2.5 mL or 3 mL) with an attached needle.
Insulin syringe(s) for drawing and injecting individual doses.
Alcohol preparation pads for sanitation.
A sharps container for safe disposal of used needles and syringes.

Step-by-Step Instructions to Reconstitute Tesamorelin 10 mg

Follow these steps carefully to ensure the peptide is mixed properly and remains stable for administration:

1. Sanitize Your Workspace

Thoroughly wash your hands with soap and water. Clean your work surface and lay out all materials. Put on sterile gloves if available, though proper aseptic technique with clean hands is also acceptable for self-administration.

2. Prepare the Vials

Remove the plastic cap from both the tesamorelin powder vial and the bacteriostatic water vial. Take an alcohol prep pad and vigorously scrub the rubber stopper of each vial for several seconds. Allow the stoppers to air dry completely to prevent alcohol from contaminating the solution. Do not touch the stoppers after cleaning.

3. Withdraw the Diluent

Using the mixing syringe, pull back the plunger to draw air equal to the amount of bacteriostatic water you intend to inject into the tesamorelin vial (e.g., 2 mL). Carefully insert the needle into the bacteriostatic water vial and inject the air. Invert the vial and withdraw the desired amount of bacteriostatic water by slowly pulling back the plunger.

4. Inject the Diluent into the Peptide

Insert the needle into the tesamorelin powder vial. Aim the needle so that the tip is directed at the glass wall of the vial, not directly onto the powder. Slowly and steadily push the plunger to inject the bacteriostatic water. This technique helps prevent foaming, which can damage the delicate peptide molecules. The vacuum seal may pull the water into the vial automatically.

5. Mix the Solution

With the needle and syringe still attached to the vial, gently roll the vial between your hands for 30 seconds. Do not shake the vial, as this can denature the peptide. Continue to roll the vial until the white powder is completely dissolved and the solution is clear and colorless, without any particles. Some formulations may take a few minutes to fully dissolve.

6. Store the Reconstituted Vial

Remove the mixing syringe and recap the vial. Immediately place the reconstituted tesamorelin vial in a refrigerator (between 2–8°C or 36–46°F), away from light. Reconstituted peptides are more fragile and have a limited lifespan compared to their lyophilized form.

Dosage Calculation for a 10 mg Vial

When reconstituting with a specific amount of bacteriostatic water, the resulting concentration will determine how to measure doses using an insulin syringe. For example, if 2 mL of bacteriostatic water is used to reconstitute 10 mg of tesamorelin, the concentration is 5 mg/mL. A 100-unit insulin syringe typically has 1 mL divided into 100 units.

Remember: Always consult your healthcare provider or prescribing clinic for specific dosage recommendations and instructions on how to measure your required dose based on the reconstitution volume you use.

Comparison of Tesamorelin Formulations

Understanding the differences between formulations is vital for correct handling and administration.


Feature	Compounded/Research (10 mg)	EGRIFTA SV (2 mg)	EGRIFTA WR (11.6 mg)
Approval Status	Not FDA-approved for human use	FDA-approved	FDA-approved
Diluent	Bacteriostatic Water	Sterile Water for Injection	Bacteriostatic Water
Concentration (after mixing)	Depends on diluent volume	2 mg/0.5 mL (4 mg/mL)	8 mg/mL (with 1.3 mL)
Reconstitution Frequency	As needed (e.g., weekly)	Daily	Weekly
Storage After Mixing	Refrigerated (2–8°C); typical use within ~30 days	Use immediately; do not refrigerate	Room temp (20–25°C); use within 7 days

Safe Injection and Post-Reconstitution Handling

After successfully reconstituting the peptide, observe the following safe practices:

Inspect the solution: Always visually inspect the reconstituted solution before each injection. It should be clear, colorless, and free of any particulate matter. If it appears cloudy, discolored, or contains solid particles, do not use it.
Rotate injection sites: For subcutaneous injections, rotate sites on the abdomen away from the navel. Avoid injecting into areas that are scarred, bruised, or hardened from previous injections to minimize irritation.
Dispose of sharps: Used needles and syringes should be placed immediately into a designated sharps container to prevent accidental needle sticks.
Protect from light: Keep the reconstituted vial protected from direct light to help maintain its stability.

Conclusion

Reconstituting tesamorelin 10 mg requires careful attention to detail and a strict commitment to aseptic technique. While not an FDA-approved formulation, this compounding or research-grade peptide can be safely mixed using bacteriostatic water and stored in a refrigerator for a limited period, typically up to 30 days. Always verify the correct diluent and volume with your prescribing clinic or provider, and be mindful of the significant differences from commercially available products like Egrifta SV or WR. By following these guidelines, you can ensure accurate dosing and maximum potency of your peptide solution. For further information and patient resources on tesamorelin, refer to authoritative sources such as MedlinePlus and Drugs.com.

Frequently Asked Questions

For a 10 mg tesamorelin vial, which is typically a compounded or research-grade peptide, bacteriostatic water for injection is the correct diluent to use. It contains a preservative that allows for multi-dose usage over a period of up to 30 days.

The amount of bacteriostatic water to add should be determined by your prescribing clinic or research provider. A common volume used is 2 mL, but this can vary depending on the desired concentration.

While sterile water is sometimes used, it is recommended to use bacteriostatic water for multi-dose peptides like a 10 mg tesamorelin vial. Sterile water does not contain a preservative, and the solution becomes susceptible to bacterial growth after the first puncture, so it should be used immediately and any remainder discarded.

The correct method is to gently roll the vial between your hands for about 30 seconds after adding the diluent. You must never shake the vial, as this can cause foaming and degrade the peptide.

After reconstitution, the vial should be stored in the refrigerator at 2–8°C (36–46°F). Keep it away from light. The solution is typically stable for up to 30 days when properly stored.

When reconstituted with bacteriostatic water and stored properly in the refrigerator, the peptide is generally stable for about 30 days. Always check with your provider for specific recommendations.

If the solution appears cloudy, discolored, or contains any particles after mixing, do not use it. The solution should be clear and colorless. This could indicate contamination or degradation of the peptide.